Graphene Quantum Dots (GQDs), a brand-new class of luminous zero-dimensional materials, due to their distinct optical characteristics, size and quantum confinement, superior biocompatibility, minimal cytotoxicity and customizable fluorescence properties, graphene quantum dots (GQDs) have demonstrated significant potential in various applications. In this current work, a low cost, green synthesis and hydrothermal approach is suggested for producing graphene quantum dots using citrus extract. The results for the UV Spectrography, X-ray Diffraction (XRD), SEM and TEM technologies serve to illustrate the structure and shape of the product offering. The antibacterial and phytotoxic properties of citrus extract are examined. The average size of GQD crystallites is 36.2 nm and with the UV absorption peak of 495.6 nm. According to the TEM data, the majority of the particle sizes were spherical and ranged in size from 50 to 150 nm. Maximum antibacterial activity was shown by citrus extract-Graphene Quantum Dots against a variety of bacterial species, including Pseudomonas aeruginosa and Escherichia coli. These findings suggested that natural alternatives may be used to research the physical and therapeutic aspects of graphene quantum dots for various applications.