A study was conducted to assess the groundwater quality in Biratnagar of Morang district of Nepal on the basis of some important physicochemical and microbiological analysis. During the study, the elevated value of turbidity ranged from 2.1 소 2.80 NTU (ward no. 8) to 81.46 소 44.28 NTU (ward no. 10), free-carbondioxide (FCO2) ranged from 12 소 2.45 mg/L (ward no. 21) to 17.2 소 3.03 mg/L (ward no. 8), arsenic ranged from nil (ward no. 3) to 0.17 소 0.21 mg/L (ward no. 1), iron (Fe) ranged from 0.45 mg/L (ward no. 7) to 3.67 mg/L (ward no. 4), manganese (Mn), ranged from 0.45 mg/L (ward no. 7) to 5.99 mg/L (ward no. 16) and fecal coliform bacteria were found positive in 10 wards (ward nos. 2, 3, 4, 9, 10, 11, 14, 15, 20 and 21) constituting 45.45% of the total samples ranging from 3 to 25 MPN/100 ml. Out of the total analyzed samples, turbidity in 78 samples (70.90%), FCO2, Fe, and Mn in all samples (100%) and arsenic in 37 water samples (33.64%) were crossed the permissible limit of WHO guideline. However, the value of pH, EC, DO, TH, PO4-P, NO3− N, Cd, Zn, and Pb were below the WHO guideline value. The status of luoride was below detectable level in all the analyzed groundwater samples.