The influence of enriching the diet of chicken with 5% of linseed oil as a vegetable source of n3 fatty acids in the form of linolenic acid on the accumulation of n3 long chain polyunsaturated fatty acids in different tissues was investigated. The fatty acid profile of the different tissues reflected dietary fatty acid profile. In general, the birds fed linseed oil showed a significant difference in the summarized value of n3 fatty acid (P< 0.001) for thigh and adipose tissue. The increase in n3 polyunsaturated fatty acids (PUFA)(P< 0.001) resulted in a significant decrease in n6 fatty acids (P< 0.001) and n6/n3 ratio (P< 0.001) in thigh and adipose tissue. The observed n6/n3 ratio in the edible tissue (thigh) of linseed oil fed birds in a prolonged feeding period was in accordance with dietary recommendations for human nutrition.