Brazilians represent one of the most heterogeneous populations in the world as the result of five centuries of interethnic crosses. We investigated the polymorphism of the HLA‐G locus in a Brazilian urban sample. HLA‐G alleles were determined by SBT analysis of exons 2–4 and 8 in 103 unrelated individuals. The genotyping analysis identified 11 different HLA‐G alleles, including the recently described G*010110 allele, and four new variants in this population. These data indicate that this Brazilian population presents high HLA‐G allelic polymorphism, suggesting increased levels of intrapopulational genetic diversity that may be because of the admixed nature of the sample. This is the first study regarding HLA‐G genotyping that was performed at such refined resolution level in this heterogeneous population.