Synthetic RNA oligos exhibit purity decreasing as a function of length, because the efficiency
of the total synthesis is the numerical product of the individual step efficiencies, typically
below 98%. Analytical methods for RNAs up to the 60 nucleotides (nt) have been reported,
but they fall short for purity evaluation of 100nt long, used as single guide RNA (sgRNA) in
CRISPR technology, and promoted as pharmaceuticals. In an attempt to exploit a single
HPLC method and obtain both identity as well as purity, ion-pair reversed-phase …