Biomolecular condensates are membrane-less cellular compartments that form via phase
separation. They serve a multitude of functions in all types of cells. Important insights into the
composition, architecture and dynamics of biomolecular condensates have been obtained
by harnessing the power of fluorescence-based technologies. In this chapter, methods will
be discussed for (1) fluorescent labelling of macromolecules,(2) spatial and temporal
mapping and tracking of target molecules in cellular and in vitro settings,(3) controlling …