Exploring plant transcriptomes using ultra high-throughput sequencing

L Wang, P Li, TP Brutnell - Briefings in functional genomics, 2010 - academic.oup.com
large number of redundant placements in complex genomes, large differences in library
populations if multiple … methods, where gene transcripts are represented by multiple alignments. …

Current-generation high-throughput sequencing: deepening insights into mammalian transcriptomes

BJ Blencowe, S Ahmad, LJ Lee - Genes & development, 2009 - genesdev.cshlp.org
Sequencing of PEs with variable intervening distances should facilitate defining how
multiple transcript … , are combined within individual transcripts. Moreover, systems currently in …

Multiplex sequencing of paired-end ditags (MS-PET): a strategy for the ultra-high-throughput analysis of transcriptomes and genomes

P Ng, JJS Tan, HS Ooi, YL Lee, KP Chiu… - Nucleic acids …, 2006 - academic.oup.com
… each full-length transcript were simultaneously extracted… high-throughput sequencing and
the accurate demarcation of transcriptional unit boundaries in assembled genome sequences

Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

Q Pan, O Shai, LJ Lee, BJ Frey, BJ Blencowe - Nature genetics, 2008 - nature.com
many of which are tissue specific. By combining mRNA-Seq and EST-cDNA sequence data,
we estimate that transcripts … ∼100,000 intermediate- to high-abundance alternative splicing …

Highthroughput sequencing for biology and medicine

WW Soon, M Hariharan, MP Snyder - Molecular systems biology, 2013 - embopress.org
… These methods not only identify RNA polymerase II‐bound transcripts but also the direction
of transcription and its rate of decay. These efforts have revealed promoter‐proximal pausing …

Analysis of HIV-1 expression level and sense of transcription by high-throughput sequencing of the infected cell

G Lefebvre, S Desfarges, F Uyttebroeck… - Journal of …, 2011 - Am Soc Microbiol
… a small component of antisense viral transcription. Of the detected cellular transcripts, 826
(2.3%… We observed very active transcription of these elements, with 1 transcript in 237 being of …

Human transcriptome array for high-throughput clinical studies

, … and Host Response to Injury Large … - Proceedings of the …, 2011 - National Acad Sciences
… examination of annotated transcripts yielded a comprehensive collection of unique transcripts
from 35,123 transcript clusters (genes). Comparisons of these unique transcripts defined a …

Overview of high throughput sequencing technologies to elucidate molecular pathways in cardiovascular diseases

JM Churko, GL Mantalas, MP Snyder, JC Wu - Circulation research, 2013 - Am Heart Assoc
… can be used to determine which mRNA transcripts are … By sequencing multiple genomes,
it is therefore, possible to … project 6 have used many of the high throughput sequencing (HTS) …

Methodologies for transcript profiling using long-read technologies

S Oikonomopoulos, A Bayega, S Fahiminiya… - Frontiers in …, 2020 - frontiersin.org
… , followed recently by the Sequel, which carries several modifications and improvements of
the original technology that result in higher throughput. The number of ZMWs increased from …

FragSeq: transcriptome-wide RNA structure probing using high-throughput sequencing

JG Underwood, AV Uzilov, S Katzman, CS Onodera… - Nature …, 2010 - nature.com
… For each site along the transcript, we counted how many reads begin there and how many
trim reads (Supplementary Notes 1 and 2) end there, summing them to get counts of observed …