Cell-sized giant unilamellar vesicles (GUVs) are an ideal tool for understanding lipid
membrane structure and properties. Label-free spatiotemporal images of their membrane
potential and structure would greatly aid the quantitative understanding of membrane
properties. In principle, second harmonic imaging is a great tool to do so, but the low degree
of spatial anisotropy that arises from a single membrane limits its application. Here, we
advance the use of wide-field high throughput SH imaging by SH imaging with the use of …