High-dynamic-range microscope imaging based on exposure bracketing in full-field optical coherence tomography

A Leong-Hoi, PC Montgomery, B Serio… - Optics Letters, 2016 - opg.optica.org
A Leong-Hoi, PC Montgomery, B Serio, P Twardowski, W Uhring
Optics Letters, 2016opg.optica.org
By applying the proposed high-dynamic-range (HDR) technique based on exposure
bracketing, we demonstrate a meaningful reduction in the spatial noise in image frames
acquired with a CCD camera so as to improve the fringe contrast in full-field optical
coherence tomography (FF-OCT). This new signal processing method thus allows improved
probing within transparent or semitransparent samples. The proposed method is
demonstrated on 3 μm thick transparent polymer films of Mylar, which, due to their …
By applying the proposed high-dynamic-range (HDR) technique based on exposure bracketing, we demonstrate a meaningful reduction in the spatial noise in image frames acquired with a CCD camera so as to improve the fringe contrast in full-field optical coherence tomography (FF-OCT). This new signal processing method thus allows improved probing within transparent or semitransparent samples. The proposed method is demonstrated on 3 μm thick transparent polymer films of Mylar, which, due to their transparency, produce low contrast fringe patterns in white-light interference microscopy. High-resolution tomographic analysis is performed using the technique. After performing appropriate signal processing, resulting XZ sections are observed. Submicrometer-sized defects can be lost in the noise that is present in the CCD images. With the proposed method, we show that by increasing the signal-to-noise ratio of the images, submicrometer-sized defect structures can thus be detected.
opg.optica.org
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