[HTML][HTML] High-speed microscopy with an electrically tunable lens to image the dynamics of in vivo molecular complexes
Y Nakai, M Ozeki, T Hiraiwa, R Tanimoto… - Review of Scientific …, 2015 - pubs.aip.org
We provide an evaluation for an electrically tunable lens (ETL), combined with a microscope
system, from the viewpoint of tracking intracellular protein complexes. We measured the
correlation between the quantitative axial focus shift and the control current for ETL, and
determined the stabilization time for refocusing to evaluate the electrical focusing behaviour
of our system. We also confirmed that the change of relative magnification by the lens and
associated resolution does not influence the ability to find intracellular targets. By applying …
system, from the viewpoint of tracking intracellular protein complexes. We measured the
correlation between the quantitative axial focus shift and the control current for ETL, and
determined the stabilization time for refocusing to evaluate the electrical focusing behaviour
of our system. We also confirmed that the change of relative magnification by the lens and
associated resolution does not influence the ability to find intracellular targets. By applying …