The doping of carbon quantum dots with nitrogen provides a promising direction to improve fluorescence performance and broaden their applications in sensing systems. Herein we report a one‐pot solvothermal synthesis of N‐doped carbon quantum dots (NCQDs) and the synthesis of a series of NCQDs with different nitrogen contents. The as‐prepared NCQDs were compared with carbon quantum dots (CQDs); the introduction of nitrogen atoms largely increased the quantum yield of NCQDs and highest emission efficiency is up to 36.3 %. The fluorescence enhancement may originate from more polyaromatic structures induced by incorporated nitrogen atoms and protonation of nitrogen atoms on dots. It was found that NCQDs can act as a multifunctional fluorescence sensing platform because they can be used to detect pH values, Ag^I, and Fe^III in aqueous solution. The fluorescence intensity of NCQDs is inversely proportional to pH values across a broad range from 5.0 to 13.5, which indicates that NCQDs can be devised as an effective pH indicator. Selective detection of Ag^I and Fe^III was achieved based on their distinctive fluorescence influence because Ag^I can significantly enhance the fluorescence whereas Fe^III can greatly quench the fluorescence. The quantitative determination of Ag^I can be accomplished with NCQDs by using the linear relationship between fluorescence intensity of NCQDs and concentration of Ag^I. The sensitive detection of H₂O₂ was developed by taking advantage of the distinct quenching ability of Fe^III and Fe^II toward the fluorescence of NCQDs. Cellular toxicity test showed NCQDs still retain low toxicity to cells despite the introduction of a great deal of nitrogen atoms. Moreover, bioimaging experiments demonstrated that NCQDs have stronger resistance to photobleaching than CQDs and more excellent fluorescence labeling performance.