Highly specific and rapid immuno-fluorescent visualization and detection of E. coli O104: H4 with protein-A coated magnetic beads based LST-MUG assay
S Barizuddin, B Balakrishnan, RC Stringer… - Journal of microbiological …, 2015 - Elsevier
A method combining immunomagnetic separation and fluorescent sensing was developed
to detect Escherichia coli (E. coli) O104: H4. The antibody specific to E. coli O104: H4 was
immobilized on protein A-coated magnetic beads. This protein-A–anti E. coli O104: H4
complex was used to bind Fluorescein IsoThioCyanate (FITC) labeled E. coli O104: H4
antigen (whole cell) on it. The goal was to achieve a fluorescently detectable protein-A–anti
E. coli O104: H4–E. coli O104: H4 complex on the magnetic beads. Fluorescent microscopy …
to detect Escherichia coli (E. coli) O104: H4. The antibody specific to E. coli O104: H4 was
immobilized on protein A-coated magnetic beads. This protein-A–anti E. coli O104: H4
complex was used to bind Fluorescein IsoThioCyanate (FITC) labeled E. coli O104: H4
antigen (whole cell) on it. The goal was to achieve a fluorescently detectable protein-A–anti
E. coli O104: H4–E. coli O104: H4 complex on the magnetic beads. Fluorescent microscopy …
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