Several studies report the influence of gender on physical exercise-induced brain plasticity, including neurotrophic factor levels, neurogenesis, and navigation strategies in spatial memory task. However, it has been noted that females are physically more active than males in animal models of physical exercise. With this in mind, we conducted an experimental study to investigate the effect of sex on the brain of rats submitted to same volume and intensity of aerobic exercise. To do so, we used calcium-binding protein parvalbumin as neuroplastic marker to explore the hippocampal formation (a brain neurogenic/mnemonic region) of male and female rats submitted to 4 weeks of aerobic exercise on a treadmill at 12 m/min, 30 min per day. Our results show that, in both sexes, physical exercise increased hippocampal density of parvalbumin neurons in the cornus ammonis (CA1, CA2/3) and hilus subfields, but not in the dentate gyrus and subiculum. No difference in exercise-induced hipocampal parvalbumin density was found between male and female rats. These findings suggest that aerobic exercise promotes similar effects on hippocampal distribution of parvalbumin neurons of male and female rats, especially when they are submitted to the same volume and intensity of physical exercise.