Context : Neuromuscular control deficit has been reported in people with chronic ankle instability (CAI) and hopping exercises have been recommended as a functional training tool to prevent lower limb injury, but its effects on lower-extremity neuromuscular control in those with CAI are unclear. Objective : To investigate the effect of hop stabilization training on neuromuscular control and self-reported function in college basketball players with CAI. Study Design : A randomized controlled trial. Setting : Research Laboratory. Patients (or Other Participants) : A total of 28 college basketball players with CAI were randomly assigned to the experimental hop stabilization group (age = 22.78 [3.09] y, weight = 82.59 [9.51] kg, and height = 187.96 [7.93] cm) or the control group (age = 22.57 [2.76] y, weight = 78.35 [7.02] kg, and height = 185.69 [7.28] cm). Intervention : Participants in the experimental group performed supervised hop stabilization exercises 3 times per week for 6 weeks. The control group received no intervention. Main Outcome Measures : Preparatory and reactive muscle activation levels and muscle onset time were assessed from 8 lower-extremity muscles during a jump-landing task before and after the 6-week training program. Results : Significant improvements in preparatory muscle activation, reactive muscle activation, and muscle onset time were noted across the lower-extremity in the experimental group relative to the control group ( P  < .05). Self-reported function also improved in the experimental group relative to the control group ( P  < .05). Conclusions : These findings demonstrate that 6 weeks of hop stabilization training is effective in improving neuromuscular control and self-reported function in college basketball players with CAI. Hop stabilization exercises can be incorporated into the rehabilitation program for CAI.