The purpose of this study is to investigate the influence of online training which consists of instructors, participants, material, method, and purpose of training on entrepreneurial motivation of MSME participants in two Super Priority Destination—Yogyakarta and Bali. This study tests a conceptual framework using a survey data of 72 MSMEs participants and employing Path Analysis approach. The result shows there is only one variable that influences the entrepreneurial motivation of MSMEs participants, that is material. Meanwhile the other variables such as instructors, participants, method, and purpose of training do not support the entrepreneurial motivation. Material of training becomes the most important factor for online training because it is held using the LMS. Previous research have discussed regarding training and motivation, but no research discussed about online training especially its effect to the entrepreneurial motivation of the MSME actors. Therefore, this study explored more about the effect of five dimension on training on the entrepreneurial motivation. This research, however, is subject to several limitations. It only surveys the online training participants in two Super Priority Destinations—Yogyakarta and Bali. The number of participants is also limited to those MSMs that are selected by the Ministry of Tourism. This study contributed in what should be prepared for making more engaging online training materials and assistance needed to keep the entrepreneurial motivation of the MSMEs actors.