Persons living with HIV infection occasionally suffer from anemia due to varying causes. These include the use of zidovudine, malnutrition especially vitamin B12 and iron deficiency, opportunistic infections by Mycobacterium tuberculosis, Pneumocystis jiroveci, and direct hematological effects of HIV infection itself within the marrow microenvironment. Persistent Parvovirus B19 (B19V) infection is a clinically important and treatable etiology of anemia in HIV-infected persons. B19V is known to show singular tropism and lytic infection of erythroid progenitor cells which may consequently result in transient red cell aplasia (TRCA) and chronic anemia. The transmission of B19V occurs mainly via respiratory droplets, but it can also be spread by contaminated blood, organ transplantation and vertical transmission [1]. After respiratory acquisition of B19V, a short replication occurs in the nasopharyngeal lymphoid tissue, followed by a massive viremia with a viral load that can exceed 1013 copies per mL [2]. Subsequently, B19V is disseminated throughout the body and enters the bone marrow microenvironment producing a generalized erythroblast infection [2]. Lymphopenia, neutropenia, and thrombocytopenia that occur during the acute viremic phase are not clinically significant [3]. The etiology of thrombocytopenia in productive B19V infection can be partly explained by the presence of viral replication in thrombocytes, without synthesis of structural proteins [3]. Patients with various types of immunosuppression may not be able to clear B19V effectively, which can result in persistent low-titer viremia accompanied by B19V related chronic anemia. Persistent B19V infection may result in severe anemia due to the inability to produce neutralizing antibodies on the conformation-dependent neutralizing epitopes, and consequent persistence of the virus replication [4]. Epidemiologically, B19V only infects humans. At 15 years of age, about 50% of children are positive for anti-B19V IgG; this figure can rise to> 90% during geriatric ages. About 25% of severe chronic anemia in HIV/AIDS patients has been attributed to B19V infection [5]. Highly active antiretroviral therapy (HAART) has been shown to stimulate reconstitution of humoral and cell mediated immune competence to various opportunistic infections [6]. Hence, B19V infection is a diagnosis of exclusion in patients who have started on HAART and developed anemia, then later not responding to empirical management [6]. However, the most common sequela of persistent B19V infection is red cell aplasia resulting in chronic or recurrent anemia with reticulocytopenia [7]. In immunocompromised individuals such as HIV/AIDS patients with chronic anemia requiring