[HTML][HTML] ISMap02 element targeted nested polymerase chain in the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples of cattle and buffaloes
M Rani, D Narang, D Kumar, M Chandra… - Veterinary …, 2018 - ncbi.nlm.nih.gov
Materials and Methods: A total of 211 fecal samples of cattle and buffaloes from different
herds around Ludhiana aged between 2 and 13 years were collected, and DNA extraction
was done from these samples. The nPCR was carried out for the detection of MAP in fecal
samples. Results: The ISMap02 element was specific for the detection of MAP only and
showed a sensitivity of detection of 7.6 fg/µL of the standard genomic DNA. Among the 211
fecal samples of cattle and buffaloes tested for the ISMap02 element, 18 samples (8.5%) …
herds around Ludhiana aged between 2 and 13 years were collected, and DNA extraction
was done from these samples. The nPCR was carried out for the detection of MAP in fecal
samples. Results: The ISMap02 element was specific for the detection of MAP only and
showed a sensitivity of detection of 7.6 fg/µL of the standard genomic DNA. Among the 211
fecal samples of cattle and buffaloes tested for the ISMap02 element, 18 samples (8.5%) …
ISMap02 element targeted nested polymerase chain in the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples of cattle and buffaloes.
MR Mamta Rani, DN Deepti Narang, DK Dinesh Kumar… - 2018 - cabidigitallibrary.org
Abstract Background and Aim: Johne's disease is chronic granulomatous enteritis which
affects ruminants. There are many diagnostic approaches for the detection of Mycobacterium
avium subsp. paratuberculosis (MAP) of which molecular detection methods using various
elements are less time consuming and more accurate. The present study was conducted
using ISMap 02 element for nested polymerase chain reaction (nPCR) based detection of
MAP in fecal samples. The aim was to test the sensitivity and specificity of the ISMap 02 …
affects ruminants. There are many diagnostic approaches for the detection of Mycobacterium
avium subsp. paratuberculosis (MAP) of which molecular detection methods using various
elements are less time consuming and more accurate. The present study was conducted
using ISMap 02 element for nested polymerase chain reaction (nPCR) based detection of
MAP in fecal samples. The aim was to test the sensitivity and specificity of the ISMap 02 …
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