Identification and removal of contaminant sequences from ribosomal gene databases: lessons from the census of deep life
Earth's subsurface environment is one of the largest, yet least studied, biomes on Earth, and
many questions remain regarding what microorganisms are indigenous to the subsurface.
Through the activity of the Census of Deep Life (CoDL) and the Deep Carbon Observatory,
an open access 16S ribosomal RNA gene sequence database from diverse subsurface
environments has been compiled. However, due to low quantities of biomass in the deep
subsurface, the potential for incorporation of contaminants from reagents used during …
many questions remain regarding what microorganisms are indigenous to the subsurface.
Through the activity of the Census of Deep Life (CoDL) and the Deep Carbon Observatory,
an open access 16S ribosomal RNA gene sequence database from diverse subsurface
environments has been compiled. However, due to low quantities of biomass in the deep
subsurface, the potential for incorporation of contaminants from reagents used during …
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