Identification of polcalcin as a novel allergen of Amaranthus retroflexus pollen
MV Moghaddam, M Fallahpour, M Mohammadi… - Allergologia et …, 2019 - Elsevier
Abstract Introduction Amaranthus retroflexus (Redroot Pigweed) is one of the main sources
of allergenic pollens in temperate areas. Polcalcin is a well-known panallergen involved in
cross-reactivity between different plants. The aim of this study was the molecular cloning and
expression of polcalcin, as well as evaluating its IgE-reactivity with A. retroflexus sensitive
patients' sera. Methods Allergenic extract was prepared from A. retroflexus pollen and the
IgE-reactivity profile was determined by ELISA and immunoblotting using sera from twenty A …
of allergenic pollens in temperate areas. Polcalcin is a well-known panallergen involved in
cross-reactivity between different plants. The aim of this study was the molecular cloning and
expression of polcalcin, as well as evaluating its IgE-reactivity with A. retroflexus sensitive
patients' sera. Methods Allergenic extract was prepared from A. retroflexus pollen and the
IgE-reactivity profile was determined by ELISA and immunoblotting using sera from twenty A …
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