Research assistants play very important role in producing research outputs and in supporting the research in HEIs. The focus of this study, initially, was to enlist the roles assigned to these teaching assistants in various setups (HEIs in this case). However, the impact of role ambiguity and role conflict on satisfaction and their intention to leave was also ascertained. Data was collected from 120 teaching assistants, engaged in various universities in Islamabad and Rawalpindi. The results found that the satisfaction and intention to leave are negatively and positively affected, respectively by these role variables. The universities are recommended to encourage the research assistants by facilitating both in term of monetary and job related factors.