Optical recording methods using voltage-sensitive dyes have proven valuable for the
analysis of neuronal networks both in vivo and in vitro. This technique detects membrane
potential changes as changes in the absorption or fluorescence of voltage-sensitive dyes
incorporated into the cellular plasma membranes. The reliability of the optical recording
technique is dependent on the dye-related response being fast enough to follow the
electrical activity and of the response being more or less proportional to the amplitude of the …