vitro were obtained from heart and tail blood of Swiss mice and from blood removed from
mosquitoes directly after feeding on these mice. In vitro produced gametocytes were
obtained from short-term cultures of the erythrocytic stages of P. berghei. Reproducible
ookinete production was obtained in medium RPMI 1640, pH 7° 8–8° 0, using in vivo and in
vitro produced gametocytes. The morphology of developmental stages of ookinetes and …