Parinari curatellifolia (Chrysobalanaceae) and Terminalia sericea (Combretaceae) have been traditionally used in Southern Highlands of Tanzania for treatment of various infectious disorders. The present study aimed to evaluate antifungal activity of the isolated compounds from Parinari curatellifolia and Terminalia sericea plant species. The ethyl acetate extract of the root barks from Parinari curatellifolia and Terminalia sericea were fractionated using column chromatography. The structures of compounds were established using both 1D and 2D-NMR spectroscopic techniques while antifungal activities of the fractions and isolated compounds were evaluated using broth microdilution assay against Candida albicans, Cryptococcus neoformans and Aspergillus niger species. Two known compounds toddalolactone (1) and 10-hydroxy-13-methoxy-9-methyl-15-oxo-20-norkaur-16-en-18-oic acid-lactone (2) from P. curatellifolia and two compounds Sericic acid (3) and sericoside (4) from T. sericea were isolated and their structures identified and confirmed by spectral data obtained and from the literatures. Strong antifungal activity was shown by Sericic acid (3) with MIC value of