Measurement of lipid composition in liver is of high importance. The J-diéerence-edited MRS allows detection of low-level overlapped metabolites, including hepatic unsaturated lipid. This study was to investigate the eìcacy of J-editing on human hepatic unsaturated lipid, with diéerent experimental setup. Multiple potential inëuencing factors such as breath control and voxel localization were parallelly compared. In-vivo hepatic MRS data was acquired in three healthy subjects. The statistical analysis of experimental variance and test re-test reliability demonstrates that MEGA-PRESS with respiratory-gating at lower level in liver allows consistent and robust in-vivo measurement of diéerent human hepatic unsaturated lipid molecules.