Salvia species are an important resource for medicinal industry. This research was conducted to develop an indirect organogenesis regeneration protocol for Salvia officinalis L. via which callus was obtained from leaf and internode explants, among these explants internode explant gave best result on MS medium supplemented with 0.5 mg/l 6-Benzylaminopurine (BAP), 2.0 mg/l 1-Naphthaleneacetic acid (NAA). The maximum percentage (70%) of regeneration was obtained with 0.5 mg thidiazuron (TDZ) from internode explants. Shootlets were highly rooted on MS/2 medium added with 1.0 mg/l indole-3-butyric acid (IBA). In vitro rooted seedlings were successfully acclimatized. This in vitro regeneration system will facilitate further development of reliable procedures for this genus.