This research was conducted during 2010 in controlled environmental conditions as two step production of wild rose rootstock. The aim of this study was to determine growth and development of wild rose at two different growth media, to preserve genotype characteristics and to shorten a multiplication time of virus-free plant material. Plants were cultivated on two different growth media. The first medium contained two hormones BAP (6-Benzylaminopurine) and IBA (Indole-3-butyric acid), and the second contained just hormone BAP. Plants that were grown on these growth media were transplanted in commercial substrate and treated with biostimulant Radifarm® by watering. Investigated parameters were shoot number, stem height, root length and stem and root mass. The results showed that addition of BAP hormone alone in the growth medium resulted in significantly longer stems, but it had no influence on shoot number. Application of the biostimulant had positive effect on shoot number and root weight. Investigation shows how the biostimulant application in R. canina transplants production improves growth and development of root and above-ground mass which is important for faster plant adaptation on stress during transplanting.