ABSTRACT Development of Various Methods in Innovative Gastroentero-Dermatology I: Does Probiotic Enema with Lactobacillus plantarum and Lactobacillus paracasei Provide Anti-Pruritic Efficacy in Dogs with Atopic Dermatitis?

Atopic dermatitis (Ad), an important dermatological problem for humans, similar to dogs. Although it is known that leaky gut-related increase in intestinal permeability and deterioration of the intestinal microbiota play a role in the disease, there are still unknowns regarding in term of both diagnosis and treatment. In this study, based on the relationship between atopic dermatitis and intestinal microbiota; 1) determination of whether Lactobacillus plantarum and Lactbacillus paracasei and probiotic enema can be used in the treatment of itching, which is the leading symptom, 2) aimed to evaluate the administration of one of the new, up-to-date probiotherapy options that are promising for role model treatment by rectal enema, also known as proctolysis, in contrast to problems of absorption in oral administration. In our study, a total of 12 dogs with Ad as different ages, breeds, and both sexes were included. Cases which had no previous treatment history, showed clinical findings compatible with Favrot criteria and atopy accompanying with increasing allergen-specific IgE level, were determined in the light of allergenspecific IgE levels (before treatment), CADESI-04 scores and clinical findings, and then rectal enema with nutraceuticals. Probiotic enema with Lactobacillus plantarum and Lactbacillus paracasei was applied 10-15 cm forward from the rectum via foley catheter or rectal catheter. Pruritus of 12 dogs with Ad applied probiotherapy by rectal enema were ceased significantly between 0 and 10 days. In conclusion, it has been observed that L. plantarum and L. paracasei strains, which can be evaluated in antipruritic lactic acid bacteria, can be used for both clinical improvement and relief of itching in dogs with atopic dermatitis at the recommended and applied dose/time in this study.