Asclepias sinaica, a host plant for many insects, was chosen for studying the patch occupancy and spatial population structure. Aphid (Aphis nerii), ladybird (Adonia variegata [Hippodamia variegata]) and ants (Monomorium carbonarium) were chosen for this investigation with the objective to test whether the incidence of these insects is associated with Asclepias sinaica plant patches. The experiment was done in Egypt during May, June and July. The temporal fluctuations of each insect species and the distribution of how Aphis nerii affects the distribution of the other two insects were also investigated. The patches were characterized by measuring plant area to assess the influence of patch quality on species occurrence. Plant use by Aphis nerii is influenced by the plant quality and inter-specific interactions. The occurrence of ladybird on plant patches was associated negatively with ant and positively with Aphis nerii distribution.