Herein, the interaction between dodecyltrimethylammonium bromide (DTAB), a cationic surfactant, and crystal violet (CV) has been studied by applying the conductometric method in aqueous solutions of short chain alcohols (ethanol, 1-propanol) and urea. The critical micelle concentration (CMC), counter ion binding (β) and the thermodynamics parameters (Δ G m 0, Δ S m 0 and Δ H m 0) for the assembly of DTAB+ CV system were determined. For the DTAB+ CV micellization in presence of aqueous alcohols and urea solutions, a single break point was obtained in every circumstance. The values of CMC for the study system are found to upsurge with the increase of concentration of alcohols and urea as well as with the increase of temperature. The Δ G m 0 values were found negative for DTAB+ CV mixtures in the study media, which indicated that the micellization process was thermodynamically spontaneous. In all cases, the-Δ H m 0 and-Δ S m 0 values disclosed that the recommended interaction forces functioning amid DTAB and CV are the hydrophobic and electrostatic interactions.