Interplay between HIV-1 infection and host microRNAs

G Sun, H Li, X Wu, M Covarrubias… - Nucleic acids …, 2012 - academic.oup.com
G Sun, H Li, X Wu, M Covarrubias, L Scherer, K Meinking, B Luk, P Chomchan, J Alluin…
Nucleic acids research, 2012academic.oup.com
Using microRNA array analyses of in vitro HIV-1-infected CD4+ cells, we find that several
host microRNAs are significantly up-or downregulated around the time HIV-1 infection peaks
in vitro. While microRNA-223 levels were significantly enriched in HIV-1-infected CD4+
CD8− PBMCs, microRNA-29a/b, microRNA-155 and microRNA-21 levels were significantly
reduced. Based on the potential for microRNA binding sites in a conserved sequence of the
Nef-3′-LTR, several host microRNAs potentially could affect HIV-1 gene expression …
Abstract
Using microRNA array analyses of in vitro HIV-1-infected CD4+ cells, we find that several host microRNAs are significantly up- or downregulated around the time HIV-1 infection peaks in vitro. While microRNA-223 levels were significantly enriched in HIV-1-infected CD4+CD8 PBMCs, microRNA-29a/b, microRNA-155 and microRNA-21 levels were significantly reduced. Based on the potential for microRNA binding sites in a conserved sequence of the Nef-3′-LTR, several host microRNAs potentially could affect HIV-1 gene expression. Among those microRNAs, the microRNA-29 family has seed complementarity in the HIV-1 3′-UTR, but the potential suppressive effect of microRNA-29 on HIV-1 is severely blocked by the secondary structure of the target region. Our data support a possible regulatory circuit at the peak of HIV-1 replication which involves downregulation of microRNA-29, expression of Nef, the apoptosis of host CD4 cells and upregulation of microRNA-223.
Oxford University Press
以上显示的是最相近的搜索结果。 查看全部搜索结果