Aspergillus niger with one-step, two-step, and spent-medium bioleaching methods. Four
effective parameters were optimized using response surface methodology (RSM) to achieve
maximum recovery of Cu and Ni. Under optimal conditions–pH of 5.15, pulp density of 10 g/l,
1E+ 007 spores of A. niger, and 4.5 days for the sample adding time− 97% and 74% of Cu
and Ni were recovered, respectively. Two-step bioleaching and spent medium bioleaching …