Investigating the effect of radiosensitizer for Ursolic Acid and Kamolonol Acetate on HCT-116 cell line
Bioorganic & Medicinal Chemistry, 2020•Elsevier
Purpose The aim of this study was evaluating the cytotoxic and radiosensitizing effects of
Ursolic Acid (UA) and Kamolonol Acetate (KA) on HCT116 cell line and finally investigating
the functional role of NF-κB and CCND1 genes in the radiosensitizing activity of UA and KA.
Materials and method The cytotoxic effects of UA and KA by MTT assay was evaluated on
HCT-116. Clonogenic assay was performed to investigate of radiosensitizing effects of UA
and KA on HCT116. To assessment the expression levels of NF-κB and CCND1 genes, real …
Ursolic Acid (UA) and Kamolonol Acetate (KA) on HCT116 cell line and finally investigating
the functional role of NF-κB and CCND1 genes in the radiosensitizing activity of UA and KA.
Materials and method The cytotoxic effects of UA and KA by MTT assay was evaluated on
HCT-116. Clonogenic assay was performed to investigate of radiosensitizing effects of UA
and KA on HCT116. To assessment the expression levels of NF-κB and CCND1 genes, real …
Purpose
The aim of this study was evaluating the cytotoxic and radiosensitizing effects of Ursolic Acid (UA) and Kamolonol Acetate (KA) on HCT116 cell line and finally investigating the functional role of NF-κB and CCND1 genes in the radiosensitizing activity of UA and KA.
Materials and method
The cytotoxic effects of UA and KA by MTT assay was evaluated on HCT-116. Clonogenic assay was performed to investigate of radiosensitizing effects of UA and KA on HCT116. To assessment the expression levels of NF-κB and CCND1 genes, real-time PCR method was used.
Results
The results of MTT assay revealed that UA and KA have cytotoxic effects on HCT116 cell line. According to clonogenic assay, survival fraction of treated cells with UA and KA has been decreased compared to the survival fraction of untreated cells. UA and KA lead to the decrease in the expression level of NF-κB. Synergistic effect of radiosensitizing agents with radiation was only approved for UA and 2 Gy of radiation.
Conclusion
Based on our study, UA and KA have cytotoxic effects on HCT116 cell line. Furthermore, UA may lead to radiosensitization of human colorectal tumor cells by NF-κB1 and CCND1signaling pathways.
Elsevier