Prospective teachers’ perception of nanotechnology, which is one of the fastest developing interdisciplinary sciences of the 21st century, is very important due to the fact that those prospective teachers are to educate the next generation. In this context, the aim of the study is to examine the perceptions of biology teacher candidates on nanotechnology. The study group; In the academic year of 2014-2015, there were 91 students who can be reached from 138 students studying at Hacettepe University Department of Biology Education. As a method of research is qualitative research method is used, the scope of the purpose of the research, the sampling method was used for sampling criteria. Biology prospective teachers' data about their perceptions about nanotechnology were obtained by using interview form consisting of two semi-structured open-ended questions. In the analysis of the data obtained from the interview questions; content analysis method was used. According to the results of the research; Biology teacher candidates' perceptions about nanotechnology are low.