The potential of insect associated bacteria regarding its enzymatic activities has raised interest, due to the special interaction between each insect species with microorganism. The aim of this study is to investigate which of the associated bacteria from Trigonopterus spp. and Imathia sp. have degrading-activity enzyme against several substrates. We had successfully isolated 21 bacteria from three species of weevils (Coleoptera: Curculionidae) comprising two Trigonopterus spp. and one Imathia sp. The weevils live on foliage of a tropical forest in Yamdena Island, Indonesia. Out of 21 bacteria, 13 isolates were characterized as Gram positive and the rest are Gram negative. The result showed that these associated bacteria were able to degrade several substrates including chitin, starch, amylopectin, CMC and tween-80. Three isolates were able to degrade three substrates. TgpA3 and TgpC10 could degrade chitin, starch and amylopectin whereas TgpC6 degrade starch, amylopectin and tween-80. Four isolates were able to degrade two substrates, TgpA2, TgpC9, TgpC11 degrade amylopectin and tween-80 while TgpB5 degrade CMC and tween-80. The remaining nine isolates showed no activity. These activities indicated that the associated bacteria have important roles to break down food for the insect host and also demonstrate its potential as enzyme source to hydrolyze forest or agricultural waste for other beneficial purposes.