Biosurfactant-producing bacteria were isolated from terrestrial and marine samples collected
in areas contaminated with crude oil or its byproducts. Isolates were screened for
biosurfactant/bioemulsifier production in different carbon sources (glucose, fructose, sucrose
and kerosene) using the qualitative drop-collapse test. Glucose produced the highest
number of positive results (17 of 185 isolates). All 17 isolates produced emulsions with
kerosene and 12 exhibited high emulsion-stabilizing capacity, maintaining 50% of the …

Biosurfactant-producing bacteria were isolated from two petroleum contaminated sites in
western Canada. Seven potential biosurfactant/bioemulsifier-producing isolates were
screened and characterized. All of the seven isolates were able to form emulsions. Emulsion-
stabilizing capacity was also measured up to 48 hrs. Strain C-111-2 and C-203-2 would lead
to highly reduced surface tension. For strain C-203-2, the optimum conditions that supported
bacteria growth and production were investigated. The influences of carbon sources …