Bacillus flexus, an alkalophilic bacterial strain, was isolated from tea rhizosphere of Golaghat District of Assam by culture enrichment method. During the study the strain was found to produce cellulose-free xylanase of molecular weight 25 kDa which was determined using SDS-PAGE analysis. The enzyme had high hydrolytic activity against birchwood xylan, oat spelt xylan and beech wood xylan; and exhibited relatively low activity against cellobiose, starch, carboxy methyl cellulose and avicel. Optimum PH and temperature in which enzyme was found active at pH 8.0±0.2 and at 70 C±2 C respectively. The Km and Vmax values were found to be 0.9 mg/ml and 3633 U/ml, respectively. Hydrolytic activity of the enzyme with metal ions were also determined and interestingly it was found that Co2+, Zn2+, Mg2+, Fe3+ and Ca2+ elevated the enzyme activity while Hg2+ and metal chelator EDTA reduced its activity.