Biopesticide formulation based on Beauveria bassiana, a wide host range insect pathogenic fungus are being marketed and used in insect pest management. These entomopathogenic hyphomycetes fungi have great potential as biological control agents against insects and in an important component within integrated pest management systems. They are being developed worldwide for the control of many pests of agricultural importance. Conodium of spore is infectious stage which attacked to the insect cuticles, germination of conodium and penetration of insect cuticles by a germ from the conodium. The insect disease caused by fungus is called muscadine disease, when the microscopic spore of fungus comes into contact with body of an insect host, they germinate, penetrate the cuticles and grow inside killing the insect within a matter of days afterwards a white mold emerges from the cadaver and produce new spores. in general 10-30% damage due to whitegrub, infestation in sugarcane crop was recorded however it is severely infested field, complex crop failure was observed, the maximum damage was observed in variety COS 88230. In the present study we have isolated Brauveria bassiaana from white grub. Conodial growth colonies were observed when grown over cadaver of white grub on Yeast Peptone Dextrose agar plate and pure culture was