Isolation of a Trypanosoma cruzi antigen by affinity chromatography with a monoclonal antibody. Preliminary evaluation of its possible applications in serological tests
CH Carbonetto, EL Malchiodi… - Clinical & …, 1990 - academic.oup.com
Clinical & Experimental Immunology, 1990•academic.oup.com
By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory,
we have isolated a T. cruzi antigen which could be useful for differential diagnosis of
Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera
from Chagas1 disease patients tested but not with sera from patients with leishmania, in
ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6
monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi …
we have isolated a T. cruzi antigen which could be useful for differential diagnosis of
Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera
from Chagas1 disease patients tested but not with sera from patients with leishmania, in
ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6
monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi …
Summary
By affinity chromatography with a monoclonal antibody (163B6), obtained in our laboratory, we have isolated a T. cruzi antigen which could be useful for differential diagnosis of Chagas' disease from leishmaniasis. This antigen, a 52-kD protein, reacted with all sera from Chagas1 disease patients tested but not with sera from patients with leishmania, in ELISA. The 52-kD antigen is widely distributed in the Trypanosoma genus since the 163B6 monoclonal antibody reacts with T. rangeli and 8 strains and a clone of T. cruzi epimastigotes.
Oxford University Press