Anti-microbial resistance is a serious and emerging crisis for both developed and developing countries throughout the world. Irrational and indiscriminate use of antibiotics flourishes the development of Multi-drug resistance (MDR) pathogens, raising some conjecture that we are almost at the verge of antibiotic era. Public hospitals play a major role in the evolving of MDR bacteria, because of frequent and excessive use of antibiotics is practiced here. The objectives of the study are the isolation of cefixime resistant Salmonella spp. from three hospitals waste samples in south-eastern region of Bangladesh (Chittagong) and the evaluation of the multidrug resistance patterns of the isolated samples. After TVC, 30 cefixime resistant Salmonella were isolated from the waste samples of three different hospitals (10 from each sample). Among the 30 isolates, 22 isolates were found resistant up to 500 μg/ml cefixime, 5 isolates showed resistance up to 400 μg/ml cefixime and rest of them were resistant up to 300 μg/ml cefixime. Isolates of cefixime resistant salmonella were further subjected to antibiotic sensitivity test by disc diffusion methods using five antibiotics eg penicillin, chloramphenicol, tetracycline, ciprofloxacin and azithromycin. Results showed that all the isolates were multi-drug resistant but all the isolates were also azithromycin sensitive. This result describes that most commercially available antibiotics are ineffective against Salmonella whereas azithromycin is still effective against Salmonella and it might be a good choice for the infections caused by Salmonella spp.