Isolation of mosquito vitellogenin genes and induction of expression by 20-hydroxyecdysone
RM Gemmill, M Hamblin, RL Glaser, JV Racioppi… - Insect biochemistry, 1986 - Elsevier
Vitellogenic female Aedes aegypti contain abundant, 6500 nucleotide long RNAs that are
not present in males or non-vitellogenic females and which were presumed to encode
vitellogenin (VG). Three clones that hybridized to cDNA made to poly (A+) RNA from
vitelogenic females, but not to cDNA made to male RNA, were selected from a genomic
library. DNA from each clone hybridized to the 6500 nucleotide RNA species. Restriction
enzyme mapping suggests the clones represent three distinct genes. The two that have …
not present in males or non-vitellogenic females and which were presumed to encode
vitellogenin (VG). Three clones that hybridized to cDNA made to poly (A+) RNA from
vitelogenic females, but not to cDNA made to male RNA, were selected from a genomic
library. DNA from each clone hybridized to the 6500 nucleotide RNA species. Restriction
enzyme mapping suggests the clones represent three distinct genes. The two that have …
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