The reactivity of flavoenzymes with dioxygen is at the heart of a number of biochemical
reactions with far reaching implications for cell physiology and pathology. Flavin-containing
monooxygenases are an attractive model system to study flavin-mediated oxygenation. In
these enzymes, the NADP (H) cofactor is essential for stabilizing the flavin intermediate,
which activates dioxygen and makes it ready to react with the substrate undergoing
oxygenation. Our studies combine site-directed mutagenesis with the usage of NADP+ …