Kinetic isotope effects in the oxidation of arachidonic acid by soybean lipoxygenase-1
C Jacquot, S Peng, WA van der Donk - Bioorganic & medicinal chemistry …, 2008 - Elsevier
C Jacquot, S Peng, WA van der Donk
Bioorganic & medicinal chemistry letters, 2008•ElsevierThe reaction of soybean lipoxygenase-1 with linoleic acid has been extensively studied and
displays very large kinetic isotope effects. In this work, substrate and solvent kinetic isotope
effects as well as the viscosity dependence of the oxidation of arachidonic acid were
investigated. The hydrogen atom abstraction step was rate-determining at all temperatures,
but was partially masked by a viscosity-dependent step at ambient temperatures. The
observed KIEs on kcat were large (∼ 100 at 25° C).
displays very large kinetic isotope effects. In this work, substrate and solvent kinetic isotope
effects as well as the viscosity dependence of the oxidation of arachidonic acid were
investigated. The hydrogen atom abstraction step was rate-determining at all temperatures,
but was partially masked by a viscosity-dependent step at ambient temperatures. The
observed KIEs on kcat were large (∼ 100 at 25° C).
The reaction of soybean lipoxygenase-1 with linoleic acid has been extensively studied and displays very large kinetic isotope effects. In this work, substrate and solvent kinetic isotope effects as well as the viscosity dependence of the oxidation of arachidonic acid were investigated. The hydrogen atom abstraction step was rate-determining at all temperatures, but was partially masked by a viscosity-dependent step at ambient temperatures. The observed KIEs on kcat were large (∼100 at 25°C).
Elsevier
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