An experiment was conducted at the Tissue Culture Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka Bangladesh from July 2011 to September 2012 to evaluate the effect of different concentrations of KIN and IAA on In vitro shoot and root initiation of tuberose. Experiment consisted seven treatments viz. T1: Simple MS, T2: MS+ 0.5 mg/L, T3: MS+ 1.0 mg/L, T4: MS+ 1.5 mg/L, T5: MS+ 2.0 mg/L and T6: MS+ 2.5 mg/L for both KIN and IAA following Completely Randomized Design with five replications. Early shoot initiation (10.1 days), leaf initiation (12.2 days), root initiation (14.92 days) was found from T3. On the other hand, maximum number of leaves (3.00/plantlet), longest leaves (5.48 cm) and maximum number of roots (3.05/plantlet) was found from T3 at 60 DAI.