LC–MS/MS for the simultaneous analysis of arachidonic acid and 32 related metabolites in human plasma: basal plasma concentrations and aspirin-induced changes …

DD Shinde, KB Kim, KS Oh, N Abdalla, KH Liu… - … of Chromatography B, 2012 - Elsevier
Eicosanoids play an important role in various biological responses and can be used as
biomarkers for specific diseases. Therefore, we developed a highly selective, sensitive, and
robust liquid chromatography–tandem mass spectrometric method to measure arachidonic
acid and its 32 metabolites in human plasma. Sample preparation involved solid phase
extraction, which efficiently removed sources of interference present in human plasma.
Chromatographic separation was performed using a Luna C8-column with 0.5 mM …
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