The present study reports the length‐weight relationships (LWRs) for 18 estuarine species sampled in Guaratuba Bay, South Brazil. For the majority of these 18 species the LWRs are known from the literature, but these estimates do not consider sexes separately which is done in this contribution. Different fishing gears were used in the samplings (cylindrical nets – 1 mm mesh size, bottom trawls – 250 mm mesh size in the arms and 12 mm mesh size in the bag, beach seine – 5 mm mesh size and hook and line fishery) between September 2007 to April 2014. The specimens were sexed and the LWRs were calculated for each sex separately. There were differences between sexes in growth type, showing the importance of sexing in these studies. We reported for the first time (based on FishBase) the LWRs for two species: Ctenogobius shufeldti and Hypleurochilus fissicornis.