Tobacco is the most profitable and main nonfood agricultural crop worldwide, from which leaves for cigarette manufacturing are obtained. Despite the proven harm of cigarettes to human health, thousands of families are financially dependent on the crop for the purpose of cigarette production. From efforts to install tobacco farming to produce biodiesel, which promote agricultural diversification, the objective of this study was to apply a life cycle assessment (LCA) from agricultural seed production to biodiesel production. Thus, the Solaris tobacco, which receives treatment that is similar to the cultivation of conventional tobacco, was evaluated. The LCA was performed in steps: tobacco seed production, oil extraction from tobacco seed and biodiesel production. The research was performed considering the functional unit of 1 kg of biodiesel produced. The SimaPro 8.5 software, which contains the Ecoinvent 3.4 database and the evaluation methods Ecological Scarcity 2006, EDIP2003, EPD 2008, BEES, CML 2, CML 2001, Eco-Indicator 99, EPS 2000, IMPACT 2002+, ReCiPe and TRACI 2, were the analytical tools. The production of Solaris tobacco biodiesel creates impacts that are similar to those that have been identified in biodiesel production from other crops. The total damage for the production of 1 kg of biodiesel from Solaris tobacco was determined to be 1.07 10^-5 Daly, 7.13 × 10^-8 species yr^-1 and $1.42 for categories related to human health, ecosystems and resources, respectively. The LCA results served as a tool to improve biodiesel production and prevent future environmental impacts. Our analysis of the LCA normalization results in each phase revealed that fertilizers and energy use were the largest contributors to the environmental impact.