One of the key questions in understanding plant development is how single cells behave in
a larger context of the tissue. Therefore, it requires the observation of the whole organ with a
high spatial-as well as temporal resolution over prolonged periods of time, which may cause
photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet
microscopy, which is characterized by mounting the plant vertically on the surface of a gel.
The plant is mounted in such a way that the roots are submerged in a liquid medium while …