Abstract Effect of five different proprietary vitamin-mineral premixes (VMP) on lipid profile of eggs from laying chickens raised under two rearing systems (RS) in the duration of storage (DOS) was undertaken. Bovan nera pullets (n= 480) at week 18 (point of lay) were divided into two equal portions of 240 birds each for conventional battery cage (BC) and an open-sided deep litter (DL) housing system respectively. Birds were randomly allotted to five dietary treatments of 48 pullets per treatment both in the BC and DL. Each treatment was replicated 6 times with 8 pullets per replicate. Five isocaloric and isonitrogenous diets were formulated, each was supplemented appropriately with 0.25% of different VMP 1, 2, 3, 4 and 5 to obtain treatments T1, T2, T3, T4 and T5 respectively. The experimental diets and water were offered to birds ad libitum. The experiment was a 2 x 5 x 5 factorial arrangement in a completely randomised design. Effect of RS on yolk total cholesterol, high density lipoprotein, and very low density lipoprotein was similar (p< 0.05). However, values of triglyceride and low density lipoprotein were significantly different (p< 0.05). All yolk lipid indices varied significantly (p< 0.05) with the type of dietary VMP as well as the interaction between RS and VMP type. The lipid peroxidation of eggs was higher (p< 0.05) in the DL (0.034) compared with the BC (0.028). Egg thiobarbituric acid reactive substances (TBARS)(mg/dL) from hens fed T1, T2, T3, T4 and T5 were 0.028, 0.031, 0.033, 0.032 and 0.027 respectively and they varied significantly (p< 0.05). Yolk TBARS for days 0, 7, 14, 21 and 28 were 0.021, 0.026, 0.033, 0.035 and 0.036 respectively also increased significantly (p< 0.05) with DOS. TBARS regression on DOS gave positive and highly significant (p< 0.01) linear equations: y= 0.00056 x+ 0.022; R2= 0.842 for eggs from DL while y= 0.00056 x+ 0.019; R2= 0.688 for those from BC. Therefore, individual effect and the interactions of VMP type, RS and DOS altered the lipid profile of eggs.