Liquid chromatographic determination of γ-aminobutyric acid in cerebrospinal fluid using 2-hydroxynaphthaldehyde as derivatizing reagent
MY Khuhawar, AD Rajper - Journal of Chromatography B, 2003 - Elsevier
gamma Aminobutyric acid (GABA) was determined by precolumn derivatization with 2-
hydroxynaphthaldehyde and elution was made using Phenomenex C18, 5 μm column with
methanol: water (62: 38 v/v) and UV detection at 330 nm. In a mixture containing glycine, l-
lysine and tyramine GABA separated completely. A number of amines and amino acids
tested did not affect the response of GABA. A linear calibration curve was obtained for GABA
in the range of 1.2–28.0 μg/ml with detection limit of 2.8 ng/injection (5 μl). The method was …
hydroxynaphthaldehyde and elution was made using Phenomenex C18, 5 μm column with
methanol: water (62: 38 v/v) and UV detection at 330 nm. In a mixture containing glycine, l-
lysine and tyramine GABA separated completely. A number of amines and amino acids
tested did not affect the response of GABA. A linear calibration curve was obtained for GABA
in the range of 1.2–28.0 μg/ml with detection limit of 2.8 ng/injection (5 μl). The method was …
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