Low abundance drug resistance variants in transmitted HIV drug resistance surveillance specimens identified using tagged pooled pyrosequencing
H Ji, B Liang, Y Li, G Van Domselaar, M Graham… - Journal of virological …, 2013 - Elsevier
HIV drug resistance (DR) testing using Sanger sequencing (SS) is limited by the inability of
the method to identify low abundance drug resistance variants. The application of tagged
pooled pyrosequencing (TPP) for HIV DR surveillance is described and the results
compared with SS. HIV+ serum specimens were genotyped using both SS and TPP.
Surveillance drug resistance mutations were identified using SS and TPP consensus reads
at multiple mixed base identification thresholds (MBITs). Drug resistance patterns were …
the method to identify low abundance drug resistance variants. The application of tagged
pooled pyrosequencing (TPP) for HIV DR surveillance is described and the results
compared with SS. HIV+ serum specimens were genotyped using both SS and TPP.
Surveillance drug resistance mutations were identified using SS and TPP consensus reads
at multiple mixed base identification thresholds (MBITs). Drug resistance patterns were …
Low abundance drug resistance variants in transmitted HIV drug resistance surveillance specimens identified using tagged pooled pyrosequencing.
JHZ Ji HeZhao, LB Liang Ben, LY Li Yang… - 2013 - cabidigitallibrary.org
HIV drug resistance (DR) testing using Sanger sequencing (SS) is limited by the inability of
the method to identify low abundance drug resistance variants. The application of tagged
pooled pyrosequencing (TPP) for HIV DR surveillance is described and the results
compared with SS. HIV+ serum specimens were genotyped using both SS and TPP.
Surveillance drug resistance mutations were identified using SS and TPP consensus reads
at multiple mixed base identification thresholds (MBITs). Drug resistance patterns were …
the method to identify low abundance drug resistance variants. The application of tagged
pooled pyrosequencing (TPP) for HIV DR surveillance is described and the results
compared with SS. HIV+ serum specimens were genotyped using both SS and TPP.
Surveillance drug resistance mutations were identified using SS and TPP consensus reads
at multiple mixed base identification thresholds (MBITs). Drug resistance patterns were …
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